| Recommended for: |
Reprogramming various cell types to iPSCs
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| Components: |
1 vial of minicircle DNA, 100 µg/vial
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Accessory Products:
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• mTeSR®1 (Catalog #05850, #05870, #05875)
• TeSR™2 (Catalog #05860, #05880)
• EasySep® hESC/hiPSC SSEA-4 Positive Selection Kit (Catalog #18165)
• AggreWell™ 400 (Catalog #27845, #27945)
• AggreWell™ 800 (Catalog #27865, #27965)
• EasySep® hESC-derived CD34 Positive Selection Kit (Catalog #18167)
• mFreSR™ (Catalog #05854, #05855)
• CryoStor™CS10 (Catalog #07930)
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| Intended Use Statement: |
For Research Use Only. Not for Therapeutic or Diagnostic Use. |
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Equipment Required:
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The Jia publication uses the Amaxa nucleofector system (machine, program and reagents) for 1 of 3 transfections performed in the protocol. This may be useful to customers but may not be critical if they can achieve sufficient transfection efficiencies and survival with alternate reagents (will have to be determined and optimised by the end user).
Access to automated cell sorting may also be helpful to customers to sort for GFP positive cells after transfection so as to ultimately calculate the frequency of iPSC generation (iPSC clones/transfected cells).
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